Objective
Research objectives and content
There have been recent important advances, both theoretical and experimental, in the description of the aqueous solutions of polyelectrolytes. On another hand, DNA condensation, a very important process in the regulation of the genetic material, is probably controlled by a delicate balance involving electrostatic interactions. We plan to describe in a quantitative manner the electrostatic interactions in DNA aqueous solutions by studying solutions with and without condensing agents (salts, surfactants). We propose to confine the solutions in freely-suspended thin liquid films, and to measure the interaction forces between the film surfaces with a device called a 'thin film balance'. These forces reflect the structure of the solution due to the electrostatic. repulsions between the polymer chains. Independent classical X-ray scattering determination of the bulk solution's structure will be performed. The eventual effects of polymer confinement close to the surfaces will then be analysed. The structure of the surface monolayers will be determined independently by other methods involving the free surface of the solution: surface tension, ellipsometry, X-ray and neutron reflectivity. Special attention will be paid to the case of condensation with cationic surfactants, in view of the potential applications for cationic carriers in gene therapy.
Training content (objective, benefit and expected impact)
I will benefit greatly from learning the experimental techniques involved in the study, in particular, ellipsometry, thin film balance, X-ray and neutron reflectivity, and scattering techniques. Furthermore, biopolymer research is a field at the boundary between physical chemistry and biology, and will probably expand considerably in the coming years. The group of Dr. D. Langevin is participating in a cooperative research effort initiated in France in this area. I therefore expect to attend severa group meetings and meet a large number of French scientists. These connections will be useful for the future research that I would like to pursue in Germany. Links with industry / industrial relevance (22)
Our project is closely connected to gene therapy research. Many industrial companies are interested in this area (in France for instance Rhtne-Poulenc). Contacts with these companies will be made in relation to the proposed project.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences biological sciences genetics DNA
- natural sciences chemical sciences polymer sciences
- engineering and technology materials engineering coating and films
- natural sciences chemical sciences physical chemistry
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
91405 Orsay
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.