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Content archived on 2024-04-30

Mammalian nuclear rna editing by adenosine deamination - a search for edited transcripts

Objective



Research objectives and content
The aim of my project is to identify transcripts edited by adenosine deamination. I plan to select transcripts that contain inosine, making use of the distinct biochemical properties of this nucleotide in comparison to guanosine When mRNA is treated with RNAse T1, the enzyme cleaves 3' to every guanosine and inosine. However, if the guanosine is modified by glyoxal treatment, the site is protected against RNAse T1. Glyoxal treatment modifies less efficiently inosine residues. This difference can be used to distinguish between RNAs containing guanosine an inosine residues.
Trainings content (objective, benefit and expected impact)
I am certain that the training necessary to carry out the project, which involve a wide range of molecular biology techniques, will contribute enormously to my following scientific career.
Links with industry / industrial relevance (22)

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

RGI - Research grants (individual fellowships)

Coordinator

Max-Planck-Gesellschaft zur Förderungder Wissenschaften e.V.
EU contribution
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Address
29,Jahnstraße
69120 Heidelberg 1
Germany

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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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