Research objectives and content
The endocytic and secretory pathways of eukaryotic cells consist of various specialised membrane-bounc compartments. Vesicular transport between these compartments is fundamental to the organisation and function of: intracellular traffic routes. This process involves specific interactions between vesicles and target membranes which ensure that only appropriate fusion events occur. These interactions are thought to be regulated in part by integral membrane proteins on vesicles and target organelles, named v-SNAREs and t-SNAREs, respectively. The discovery of a novel t-SNARE on an early endosomal compartment in yeast allows us to investigate the recycling of proteins from the endocytic to the secretory pathway. The genetic and biochemical studies described in this proposal offer a unique opportunity to identify the components needed for recycling of proteins to the Golgi apparatus and cell surface, and for targeting of late secretory vesicles to particular sites of the plasma membrane The proposed studies should lead to a better understanding of how eukaryotic cells maintain their compartmental organisation.
Training content (objective, benefit and expected impact)
The requested training should deepen my insight into the organisation of the intracellular membrane system, and the traffic routes between organelles. The host institution offers me a unique chance to broaden my research skill in this field, learn yeast genetics, and gain training in a broad variety of innovative techniques.
Links with industry / industrial relevance (22)
No links with industry / no industrial relevance