Objective
Research objectives and content
Despite a wealth of data coming from in vitro transcription and transfection experiments the role of TFIID subunits (TAFIIs in trancriptional regulation in mammalian cells has not been strictly defined. I propose to use mammalian genetic techniques to address this question. Previous studies have implicated TAFII28 as a cell-specific TAFlI involved in transcriptional regulation by nuclear receptors. I will determine the mRNA and protein expression pattern of TAFII28 in established cell lines and in the developing and adult mouse. According to the results of this study, I will use the technique of conditional cre/lox mediated homologous recombination to inactivate TAFII28 either in F9 embryonal carcinoma cells or in ES cells allowing the generation of TAFII28 null mice. The phenotype of the resulting TAFII28 null F9 cells and/or mice will be characterised. Training content (objective, benefit and expected impact)
The research I propose will enable me to learn important genetic techniques for the study of genes in mammalian cells and techniques in biochemistry. At the IGBMC I will gain important experience in the field of transcriptional regulation, embryology, and in the biology of nuclear receptors. The proposed research entails the use of homologous recombination in F9 cells, a procedure in which the IGBMC has considerable experience. I consider that the opportunity to learn these techniques at an outstanding institute such as the IGBMC is fundamental for my research carreer.
Links with industry / industrial relevance (22) None
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
67404 ILLKIRCH GRAFFENSTADEN
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.