Objective
Research objectives and content
The aim of this project is to understand at the molecular level, how a redox, fixed nitrogen and energy-sensing protein (NIFL) responds to intracellular signals and communicates this information to a transcriptional activator (NIFA), to modulate gene expression. The role of a conserved motif in NIFL, present in a large family of proteins from Bacteria, Archaea and Eucarya which sense oxygen, redox status or light will be identified and the role of nucleotides in the activation of NIFL will be determined. A genetic approach utilising site-directed mutagenesis and positive selection for mutant phenotypes will be employed to identify regions of NIFL and NIFA required for communication. This will be complemented by a biochemical approach in which evidence for protein: protein interactions will be sought. I will also investigate the mechanism whereby NIFL inhibits transcriptional activation by NIFA.
Training content (objective, benefit and expected impact)
This project will considerably extend my existing experience which is based largely in the area of molecular genetics. I wish to develop and pursue the more biochemical and molecular biological approaches which are offered by this project in order to gain an understanding of protein structure and function as well as the molecular mechanisms involved in regulation. This training will enable me to develop the essential skills and expertise necessary to initiate my own independent research in the future.
Links with industry / industrial relevance (22)
Several biotechnology companies have expressed interest in the Framwork IV Biotechnology network: TASTES (Transcription Activation and Sensory Transduction: Elucidation of Structures) which is coordinated by the host institute.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences molecular biology molecular genetics
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics nucleotides
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NR4 7UH Norwich
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.