Objective
Research objectives and content Nucleocytoplasmic transport occurs through the nuclear pores, large macromolecular assemblies which punctuate the nuclear envelope. Cytosolic factors play an active role as import receptors both in the recognition of the protein to be imported and in the translocation through the pore in conjunction with nucleoporins. The recent discovery of export receptors mediating both protein and RNA export indicate that import and export are intimately related. In the host laboratory I intend to collaborate with Prof. Bertil Daneholt to elucidate the mechanism of premessanger RNP export using the Balbiani Ring (BR) particles as model system. A detailed structural and functional study will be carried out using the giant salivary glands of C. tentans as an in vivo experimental system. Electron microscopy will be the main technique to elucidate the export pathway of the BR particles. In particular the translocation process through the nuclear pore will be analyzed and where applicable three dimensional reconstruction by electron tomography will be applied. Training content (objective, benefit and expected impact) During my stay in B. Daneholt's laboratory, I expect to learn more about the mechanisms involved in export of macromolecular assemblies, here exemplified by the BR pre-mRNP particle. Furthermore, I will be able to combine molecular biology techniques with advanced ultrastructural analysis, and I will get acquainted with the microinjection approach in studies of export. Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences molecular biology
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Coordinator
54006 Thessaloniki
Greece
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