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Structural and functional studies of premessanger rnp transport through the nuclear pore

Objective



Research objectives and content Nucleocytoplasmic transport occurs through the nuclear pores, large macromolecular assemblies which punctuate the nuclear envelope. Cytosolic factors play an active role as import receptors both in the recognition of the protein to be imported and in the translocation through the pore in conjunction with nucleoporins. The recent discovery of export receptors mediating both protein and RNA export indicate that import and export are intimately related. In the host laboratory I intend to collaborate with Prof. Bertil Daneholt to elucidate the mechanism of premessanger RNP export using the Balbiani Ring (BR) particles as model system. A detailed structural and functional study will be carried out using the giant salivary glands of C. tentans as an in vivo experimental system. Electron microscopy will be the main technique to elucidate the export pathway of the BR particles. In particular the translocation process through the nuclear pore will be analyzed and where applicable three dimensional reconstruction by electron tomography will be applied. Training content (objective, benefit and expected impact) During my stay in B. Daneholt's laboratory, I expect to learn more about the mechanisms involved in export of macromolecular assemblies, here exemplified by the BR pre-mRNP particle. Furthermore, I will be able to combine molecular biology techniques with advanced ultrastructural analysis, and I will get acquainted with the microinjection approach in studies of export. Links with industry / industrial relevance (22)

Coordinator

Aristotle University of Thessaloniki
Address
Analytical Chemistry Lab
54006 Thessaloniki
Greece