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Content archived on 2024-04-30

Simple sequence repeats - ssrs - in relation to adaptation in wild barley

Objective



Research objectives and content
This is an exciting and innovative project in which I have the opportunity to extend my training and knowledge in the application of molecular markers to the genetics of plant adaptation. The ability of simple sequence repeats to open up genomes has revolutionised research in human and mammalian genetics and will have considerable impact in the plant sciences. This project aims to exploit the recent development of SSRs in barley, to select a set of SSRs which show even genome coverage, and to apply these in genetic characterisation of a set of wild barley populations, for which between and within ecogeographic data are available. The aim being to find markers for genes associated with adaptation, and to examine whether SSRs are biologically neutral. A comparison of synteny between wild and cultivated barley responses to stress will be carried out by experimental testing of barley doubled haploid populations derived from cultivated/cultivated, cultivated/wild and wild/tild crotses. Training content (objective, benefit and expected impact)
I will receive training in the development, mapping and hands-on analyses of SSRs. Experimental data will be analysed using various statistical programmes for which formal training is given this will involve analysis of variance and regression, principal co-ordinates and principal components. Appropriate programmes will be used for genetic mapping of SSRs and mapping phenotypic/quantitative trait loci data. I will receive training in hydroponics and cold chamber experiments in examining various physiological responses to imposed stresses. I will produce a doubled haploid population from a wild barley/wild barley cross using the recent published isolated barley microspore protocol. I will be working in a multi-disciplinary team in a lively, active and exciting department. The results of this research training project will be prepared and submitted by me as a doctoral thesis.
Links with industry / industrial relevance (22)
The development of SSRs at the SCRI, and other EU partners, is geared towards improving the efficiency of breeding barley for malting quality and disease resistance. Genetic markers for abiotic stress tolerance are also being developed for application in barley improvement programmes. In addition to marker assisted selection, accelerated breeding programmes rely on the rapid production of homozygous lines for which microspore culture is a key emerging technology. Microspore culture is also seen as being a major target for cereal transformation since plants can be regenerated directly from single cultured (transformed) cells. I will therefore receive training in areas pertinent to modern plant biotechnological industries.

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Coordinator

Scottish Crop Research Institute
EU contribution
No data
Address
Invergowrie
DD2 5DA Dundee
United Kingdom

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Total cost

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