Objective
Research objectives and content
The mechanism of solute translocation across biological membranes is a fundamental and unresolved problem of modern biology. I intend to contribute to this field by investigating the structure/functic relationship of the arabinose-H+ symporter from Escherichia cold and the mj1560 multidrug efflux protein from Methanococcus jannaschii. Both proteins are already successfully over expressed in E. cold and canbe purified in satisfactorily amounts necessary for structure determination. Structure elucidation will be performed mainly by crystallization of the proteins in three dimensions followed by X-ray diffraction, but perhaps also in two dimensions followed by electron diffraction. If time permits, structure/function relationships of the arabinose-H+ symporter will be studied using fusions between this protein and the homologous galactose-H+ symporter GalP from E. coli.
Training content (objective, benefit and expected impact)
With the training in three-dimensional crystallization techniques and the use of other alternative strategic,(e.g. two-dimensional
crystallization), the proposed stay in the laboratory of Prof. Henderson will be a natural and very attractive complementation of my existing experimental skills. It will be necessary to have these skills in order to tackle in future the major unresolved problem in the field of membrane biology i.e. the mechanism of solute translocation across biological membranes.
Links with industry / industrial relevance (22) The host institution has support from Smithkline-Beecham,Glaxo-Wellcome and Sanofi for the delevelopment of antimicrobials in related research projects.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences biological sciences biochemistry biomolecules proteins
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LS2 9JT Leeds - West Yorkshire
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.