Skip to main content

Role of the angiopoietin system in tumor angiogenesis

Objective



Rescarch objectives and content
Tumors are usually not angiogenic at the beginning of their development. and conseauentely they remain limited to small volumes. The acquisition of the angiogenic activity depends on the balance of positive and negative angiogenic factors released by the tumors. The aim of this project is the identification and characterization of the molecular events that are involved in the onset of tumor angiognenesis. All the studies will be performed using as a model a transgenic mouse line expresses SV-1O large T-antigen (Tag) under the control of the rat insulin promoter (Rip1 Tag2). These mice develop tumors in the Lanoherans acells in a predictable manner and different stage of tumor progression are clearly distinguishable, thus, make them a valuable tool to study the molecular mechanism of tumor angiogenesis.Expression and functional studies of the TIE-2 receptor and its ligands angiopoietin-1 and angiopoietin-2 during tumor progression in Rip1Tag2 transgenic mice will be performed. The long term goal of the project is to determine the role of angiopoietins in tumor angiogenesis in vivo. In order to reach the aim we will generate transgenic mouse lines that overexpress angiopoietin-1 or anoiopoietin-2 specifically in the ,B cells under the control of the insulin promoter. In case the forced expression of angiopoietin has no impact on the development of islets of Langherans. these mouse lines will be crossed to Rip tag2 transgenic mice and potential effects on a cell tumorigenesis will be analyzed ..Training content (objective,benefit and expected impact) The aim of this project is the identification and characterization of the molecular events that are involved in the onset of tumor angiogenesis. In particular. I propose to investigate the functional role of TIE-2 receptor and its known ligands, angiopoietin-1 and angiopoietin-2. in tumor angiogenesis in RiplTag2 transgenic mice. Furthermore. I plan to use angiopoietin-1 and angiopoietin-2-specific probes toe identify and clone novel members of the angiopoietin family of growth factors. Links with industry/ / industrial relevance (22)

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

FORSCHUNGSINSTITUT FUER MOLEKULARE PATHOLOGIE GMBH
Address
Dr. Bohr-gasse 7
1030 Wien
Austria