Research objectives and content
Little is known on the role of the Msx genes in the developing central nervous system (CNS), although previous studies have reported expression of these genes in a number of sites of the CNS in the mouse.This project consists in the study of the detailed patterns of expression of Msxl and Msx2 genes (using knocked-in reporter genes) in the developing mouse embryos and in postnatal mice. This first task will allow us to investigate the altered zones of the developing brain by mean of several approaches such as general histology techniques (e.g. immunocytochemical analysis of developing neurons), study of apotosis alterations and description of connections. We will further investigate systematically alterations in the CNS of simple and double mutants. The innovative and important aspects of this project consist in the analysis of intact tissues from the double mutants using organotypic culture slices for a long time after birth.
Training content (objective, benefit and expected impact)
| The aims of this proposal are double. First, it can lead to a better understanding of the function of Msx genes in the developing brain and therefore might provide insights into our knowledge of the human brain congenital malformations. Second, this project will constitute for the applicant a good continuation of the study of the mechanisms of development in vertebrates. Also, it can benefit to the two institutions through an exchange of techniques and experiences.
Links with industry / industrial relevance (22)
The receiving group has established preliminary contacts and discussions with the industries Merck and Pierre Fabre Iberica in order to conduct collaborative research on genes involved in developmental malformations of the cerebral cortex.