Research objectives and content
The main objective of this project is the development of a complete kinetic model for energy transfer between individual chromophores in the higher plant Light Harvesting protein CP29 as a representative biological structure for the basic mechanism of energy transformation. This is based on two recent devlopments: (a) the possibility of producing recombinant pigment-binding proteins indistinguishable from the native proteins extracted from leaves and (b) the improvements in ultrafast spectroscopical techniques required to study the complex energy transfer and pigment coupling in this antenna complex. Mutational analysis of the CP29 protein has allowed identification (and deletion) of chlorophyll binding sites allowing a detailed spectroscopical study of recombinant CP29 and of the individual mutants each lacking a single chlorophyll binding site. We will also produce heterodimeric complexes for elucidation of the
protein-protein energy transfer.
Training content (objective, benefit and expected impact)
This training content will extremely good for the applicant. The applicant has been trained in biochemistry and basic biophysics during pre-doc and PhD work. The interfacing of Molcular genetics, Biophysics and Biochemistry, required in this project will be the best opportunity for the formation of young scientist, two combine three usually separated areas. This training obtained in broad areas of fine-resolved spectroscopy, molecular biophysics and photobiophysics will enable the applicant to substantially widen her scope which is a prerequisite for future work in a highly interdisciplinary area like photosynthesis.
Links with industry / industrial relevance (22)