Objective
Training objectives and contentMyft-5 is the first myogenic regulatory factor (MRF) expressed during mouse embryogenesis and plays a central role in skeletal muscle determination. Myf-5 is initially expressed in the most dorsal and medial cells of the dermamyotome, the location of the epaxial muscle precursors. Transgenesis experiments have shown that myf-5 has a separate enhancer for each of the anatomically distinct muscle precursor regions in the embryo. Deletion of a 2.6kb segment close to the adjacent MRF4 gene eliminates expression of myf-5 in the epaxial domain, while not altering any other aspect of the pattern.
We project to: -Investigate whether this 2.6kbsegment is sufficient for epaxial expression and define the minimal DNA sequences required. -Define protein-binding sites in the minimal region and test their function by mutagenesis and transgenesis. Characterise the proteins required for enhancer function. -Test putative signalling molecules involved in myogenesis by crossing epaxial enhancer-reporter constructs with the appropriate knockouts. -Use constructs containing both myf-5 and MRF4 genes, and carrying two reporter genes to investigate whether there are sequences that regulate both MRF genes. Training content (objective, benefit and expected impact). My expertise is in Cellular Biology and in vitro techniques. This project involves an extensive training in transgenesis, mouse breeding, micro-dissection and Developmental Biology. Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences developmental biology
- medical and health sciences clinical medicine embryology
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
SW3 6JB London
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.