Objective
Research objectives and content
Synaptic transmission in the mammalian central nervous system has been analysed traditionally via electrophysiological recordings obtained from the neuronal cell body. Using this methodology, the funtional diversity of individual synapses cannot be sufficiently described. Here, different methods suited to monitor events at the single synapse level will be applied to rat hippocampal neurons.Loose patch recordings of spontaneous miniature postsynaptic currents, so far succesfully employed for the analysis of individual excitatory synapses, will be extended to inhibitory synapses. Therby, precise informations concerning principal parameters of these synapses can be obtained; as, for example, the extent of receptor saturation by a quantum of neurotransmitter.While loose patch recordings mainly focus on the postsynaptic site, also presynaptic events will be monitored at single synapse resolution. The distribution of the synaptic vesicle protein synaptotagmin and potentially of other presynaptic proteins before and after the induction of different forms of synaptic plasticity will be followed in real time with various fluorescent labelling methods. These experiments may reveal plasticity-related changes in the protein composition of individual synapses.
Training content (objective, benefit and expected impact)
Training will be devoted to new and powerful electrophysiological and imaging techniques developed by the host laboratory. Adding to already present experience in the characterization of synaptic proteins, learning those methods and discussing state-of-the-art concepts of synaptic transmission will significantly strengthen personal expertise. Links with industry / industrial relevance (22)
The host laboratory of Dr. Malgaroli has a long-standing collaboration with Glaxo Geneva.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences neurobiology
- natural sciences biological sciences biochemistry biomolecules proteins
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
20132 MILANO
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.