Objective
Research objectives and content
Geminiviruses infect monocotyledonous and dicotyledonous plants. They can replicate in the host cells influencing their cell cycle . The wheat dwarf geminivirus codes for two proteins Rep and RepA. Rep binds to a recently identified plant Retinoblastoma protein (Rb). Rb is known as key regulator of the cell cycle in mammalian cells, where it interacts with a variety of proteins. The plant cell cycle is thought to have similar components as the mammals, but only a few of them are identified. Plants have a different developmental concept as compared to animals and these differences could be reflected on the cellular and molecular level. The project has two objectives: (A) The identification of new proteins involved in the plant cell cycle by using the plant Rb in a yeast-two-hybrid system to find interacting proteins (B) The study of the influence of the viral proteins Rep and RepA on the plant cell cycle. Rep or RepA will be introduced into synchronized wheat cell cultures. Its effects will be observed monitoring the expression on protein and RNA level of newly identified proteins (from part A) as well a of already established markers of the cell cycle. These markers are for example proliferating cell nuclear antigen (PCNA), cyclin dependent kinase (CDK) and Rb itself.
Training content (objective, benefit and expected impact)
The host laboratory has expert knowledge on the molecular biology of geminiviruses and the viral strategies used to infect susceptible plants. It is one of the two laboratories having identified the first Retinoblastoma proteins from plants. It provides experience on handling tissue culture systems of monocotyledonous plants, which will be used as model system in the project. This is an excellent basis to study effects of viral infection on the plant cell cycle. Moreover, the laboratory is a part of a large research center harboring researchers from various fields of plant and animal biology, allowing a broad exchange of information. Links with industry / industrial relevance (22)
The Friedrich Miescher-Institut (place of PhD) is a non-profit research institute belonging to Novartis, Switzlerland.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences zoology mammalogy
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
- natural sciences biological sciences molecular biology
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
28049 MADRID
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.