Regulation of interactions between ionotropic glutamate receptors and the cytoskeleton

Project Information

Grant agreement ID: FMBI983426

Project closed

Start date 1 August 1998

End date 31 July 2000

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

Centre National de la Recherche Scientifique
France

Objective

The project concerns the analysis of the spatio-temporal organisation of ionotropic glutamate receptors on the cell surface. These receptors show a highly organized membrane distribution. It has been established that the cytoskeleton plays a key role in the anchoring of these receptors but detailed knowledge of the receptor - cytoskeleton interactions, the dynamical behaviour and functional meaning still lack. With an original combination of molecular biology, fluorescence video microscopy, laser optics and electrophysiology we will study in real time the movement of individual receptors, their interaction-strength with the cytoskeleton and functional properties under different experimental conditions. We will dissect the underlying mechanisms of receptor anchorage to the cytoskeleton by studying the behaviour of mutants of both receptors and proteins that take part in the anchoring,as well as the effects of biochemical stimulations known to affect anchoring. We will characterize the redistribution of glutamate receptors during development and after the induction of synaptic plasticity. We will study the relation between receptor mobility and the functional properties of the ionotropic receptors under different anchoring regimes. The ultimate goal of these investigations is to understand the functional role of the ionotropic glutamate receptor - cytoskeleton interactions.
Training content (objective, benefit and expected impact)
With the combination of techniques offered by the host lab we will study glutamate receptor-cytoskeleton interactions. We will follow in real time the following functionally meaningful parameters: receptor mobility, receptor localization and electrical properties of the ionotropic receptors. We will study fast modulations in the interactions, which is of relevance in many interesting experimental paradigms. The expertise of the host lab in molecular biology provides the tools essential for unraveling underlying mechanisms in molecular detail. These facilities in combination with the applicants experience in patch clamping and single cell calcium imaging should provide an exiting project. We expect that this original combination of techniques offers a general approach for future studies of membrane protein - cytoskeleton interactions.
Links with industry / industrial relevance (22)
None

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.

• natural sciences › chemical sciences › inorganic chemistry › alkaline earth metals
• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › physical sciences › optics › microscopy
• natural sciences › physical sciences › optics › laser physics
• natural sciences › biological sciences › molecular biology

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Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL11 - Neurosciences (inc. Psychology)

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

Centre National de la Recherche Scientifique

• EU contribution: No data
• Address: 146,Rue Léo Saignat ; 33076 Bordeaux ; France
• Total cost: No data