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Content archived on 2024-06-10

The role of creb binding protein cbp in nuclear calcium-activated transcription

Objective



Research objectives and content Calcium is the primary mediator of electrical-dependent gene expression in neurons. It acts in two cell compartments, the nucleus and the cytoplasm, stimulating distinct mechanisms of transcriptional cont The transcription factor CRE binding protein CREB and its coactivator CREB binding protein CBP, which is recruited to the promoter by CREB phosphorylation on serine 133, are important mediators of calcium- regulated gene expression. The aim of this project is to investigate the role of CBP in calcium-induced transcription. I will identify calcium-regulated transcriptional activation domains of CBP and determine the role calcium/calmodulin dependent (CaM) protein kineses and MAP kineses, that are activated by increased intracellular calcium concentrations, in the control of their activity. Furthermore, I will analyse phosphorylation of CBP following activation of calcium signalling pathways and I will determine the role' putative phosphorylation sites in the control of CBP activity. Training content (objective, benefit and expected impact) The proposed project will allow me to acquire knowledge and expertise in calcium signalling. I will of training in studying mechanisms controlling gene expression and learn new up-to-date techniques whichwill greatly improve my scientific skills. This will, in the future, allow me to carry out independent research in molecular neuroscience. Links with industry / industrial relevance (22)

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Funding Scheme

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RGI - Research grants (individual fellowships)

Coordinator

MRC Laboratory of Molecular Biology
EU contribution
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Address
Hills Road
CB2 2QH Cambridge
United Kingdom

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Total cost

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