A structural investigation of the pigment binding sites in cp29, a minor antenna protein in higher plants

Project Information

Grant agreement ID: FMBI983497

Project closed

Start date 1 January 1999

End date 31 December 2000

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

Università degli Studi di Verona
Italy

Objective

Research objectives and content Mutants of the plant light-harvesting protein CP29 will be generated, in order to identify and alter those residues hydrogen-bonding the prosthetic groups (chlorophylls). will be principal objective is to define the spatial orientation of each chlorophyll molecule, based on the atomic model of the related light-harvesting protein LHCIIb, and to determine the effect of hydrogen-bonding on spectroscopic properties of chlorophylls. Furthermore the mutants generated, along with a further set of mutants in which the strength of hydrogen-bonding interaction has been modified for one or more of the bound chlorophylls, will be used (in collaboration with a second European research group, led by Prof van Grondelle) to study excitation and energy transfer parameters. As well as the direct, structural information this will give on CP29 and related plant light-harvesting proteins, this will prove an invaluable model system in which to determine the factors effecting energy transfer in chlorophyll-proteins generally. Training content (objective, benefit and expected impact) - I will be trained in molecular biological techniques for the generation of recombinant and mutated polypeptides in E. coli, and in reconstitution of LHC polypeptides. I will also extend my current (limited) biophysical skills to in clude such specialised areas as pump-probe spectroscopy and fluorescence line narrowing. Links with industry / industrial relevance (22)

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.

• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › physical sciences › optics › spectroscopy

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Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants

Call for proposal

Data not available

Funding Scheme

Data not available

Coordinator

Università degli Studi di Verona

• EU contribution: No data
• Address: Strada le Grazie ; 37134 Verona ; Italy
• Total cost: No data