Objective
Research objectives and content Little is known about actin nucleation in non-muscle cells, a process usually associated with membranes. It is often induced by signals from outside the cell and some phosphoinositides, especially PIP, PIP2 and PIP3 seem to be important. Moreover, many actin binding proteins can bind these lipids. Recently a simple in vitro assay was developed by the Griffiths group to study actin nucleation on purified phagosomal membranes. Actin nucleation in this system is dependent on PIP2 and on peripheral membrane proteins that are removed by 'high salt' treatment. This process can be reconstituted by adding 'high salt' extracts to salt-stripped phagosomes. Further, a correlation was found between the age period in which phagosomes can fuse (in a separate assay) and that in which they best nucleate actin. I propose to identify and characterize the protein(s) which nucleate(s) actin on phagosomal membranes and to try to correlate its (their) function with the fusion process. Training content (objective, benefit and expected impact) At the EMBL I can learn and apply the use of an elegant system that is highly versatile to study membrane-actin interactions as well as membrane fusion in a (for me) new field. Links with industry / industrial relevance (22) A deeper understanding of these processes is a fundamental cell biological problem that is important for all pharmaceutical companies.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences biochemistry biomolecules lipids
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.