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Molecular mechanisms of intracellular protein transport in mammalian cells

Objective



Transit between intracellular compartments include sorting of proteins and lipids, formation of a carrier coated vesicle, recognition and fusion of visiting fellows from different European countries to spend a training period of 2-3 years in one of the laboratories of the network. The wide this vesicle with the target membrane. In recent years, small GTPases of spectrum of topics and strategies employed to carry out the proposed the ARF and Rab families have emerged as important players of the membrane project will allow visiting scientists to learn a combination of cellular traffic machinery. While ARF proteins function in coat assembly and (e.g. use of in vitro reconstituted transport assays) and molecular disassembly, Rab proteins are implicated in the process of vesicle biology (site-directed mutagenesis and expression of mutants) techniques docking/fusion. Moreover, phosphorylation is also implicated in regulating as well as biochemical purification (purification of recombinant proteins membrane traffic. The research objectives described in this proposal focus and search for interacting factors) and genetic screening (yeast two hybrid on the functional mechanism of these proteins as well as other factors implicated in the regulation of intracellular protein transport. Seven system) methods groups from six different European countries will concert their efforts. Three laboratories of the network come from less-favoured countries. It aiming at: is crucial that these groups are funded in order to support their research activities.

At the same time, these groups have proven to have the:
1. Reconstituting different steps of intracellular transport in secretion and endocytosis in vitro in order to provide experimental systems amenable necessary know how and facilities to support training of visiting EEC fellows. Funding is rate limiting for their successful developments to biochemical manipulations. Given the present boom of interest in intracellular traffic, the visiting;
2. Studying how these transport reactions are modulated through the analysis of several factors implicated in vesicle budding, targeting, fellows will benefit of the participation to scientific meetings of high docking and fusion including the small GTPases ARF and Rab, the novel G international reputation and practical courses. Finally, the groups of protein Gh/transglutaminase, BFA-dependent ADPribosylated factors, SNAREs, this network have been involved in true collaborations in the past (see H. Stenmark, A. Valencia, O. Martinez, O. Ullrich, B. Goud and M. Zerial and substrates of protein kinases (PKC, PKA). EMBO J., 13, 575-583, 1994) and are keen in continuing the exchange of3. Addressing the functional mechanism of the regulatory factors information, expertise, technical progress and personnel in order to participating in these processes, by identifying interacting components and downstream effectors. This work will hopefully unravel the link rapidly achieve the proposed research goals existing between the machineries which control vesicle budding and vesicle targeting. These studies are expected to provide a better understanding of both the organisation of the intracellular traffic routes and the molecular mechanisms underlying vesicular transport.

Funding Scheme

NET - Research network contracts

Coordinator

European Molecular Biology Laboratory
Address
1,Meyerhofstraße
69117 Heidelberg
Germany

Participants (7)

Consorzio Mario Negri Sud - Centro diRicerche Farmacologiche e Biomediche
Italy
Address
Via Nazionale
66030 Chieti - San Maria Imbaro
Institut Curie
France
Address
26,Rue D'ulm
75231 Paris
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
Germany
Address
Pfotenhauerstr.
01307 Weissig - Dresden
National Public Health Institute
Finland
Address
166,Mannerheimintie
00330 Helsinki
The Norwegian Radium Hospital
Norway
Address
Montebello
0310 Oslo
UNIVERSITA DEGLI STUDI DI NAPOLI FEDERICO II
Italy
Address
Via Sergio Pansini 5
80131 Napoli
UNIVERSITY COLLEGE CORK, NATIONAL UNIVERSITY OF IRELAND, CORK
Ireland
Address
Lee Maltings, Prospect Row
30 Cork