DNA hybridisation has the potential to detect target sequences in a sample, this has application in identification of pathogens and viral or genetic disorders. Conventional approaches for monitoring DNA hybridisation often involve use of a fluorescent label. We propose to develop a miniaturised micro array system using impedance and surface plasmon resonance (SPA) interferometry for a label-free method of identification of pathogens in the food and environmental sectors. Impedance based measurements will be carried out on a DNA biochip composed of a 4x4 element array interdigitated electrodes and heater elements. SPR interferometry information is obtained by creation of an interference pattern and allows micro array sensing. The DNA biochips will be incorporated into a micro fluidic system and allow automated monitoring of DNA hybridisation and discrimination of alleles.
Funding SchemeEAW - Exploratory awards
666 014 Tisnov