Objective The major part of the programs of fine structure analysis of the human genome is mainly dealing with direct DNA sequencing, which involves a number of rather complicated manipulations including loading samples onto a gel and reading sequences after separation of DNA fragments. Electrophoresis is the bottle neck for large scale analysis. This project will explore a method for DNA sequencing based on the hybridization of the DNA fragment of interest to every oligonucleotide of a complete set (i.e.all possible sequences of a given length). The suggestion is to immobilise each of the oligonucleotides at an individual dot of a2-D matrix, thus allowing all the hybridizations to be processed in parallel, with the DNA fragments serving as a probe. This procedure requires a reduction of the influence of base composition and sequence on the stability of short DNA hybrids (to be able to discriminate fully annealed hybrids from hybrids with mismatches). Our approach consists of designing modified oligonucleotides that will form hybrids with DNA sequences, whose stability will not depend on base content, but only on hybrid length and presence of a mismatch. Suitable modifications, solid supports, sample preparation methods and subsequent automated software analysis will be explored. Applications will not only include fast large scale DNA sequencing, but also fast identification of genetic variability, e.g. diagnosis of genetic and infectious diseases. Fields of science natural sciencescomputer and information sciencessoftwarenatural sciencesbiological sciencesgeneticsDNAmedical and health scienceshealth sciencesinfectious diseasesnatural scienceschemical scienceselectrochemistryelectrophoresisnatural sciencesbiological sciencesgeneticsgenomes Programme(s) FP3-BIOMED 1 - Specific research and technological development programme (EEC) in the field of biomedicine and health, 1990-1994 Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator Appligene SA Address Route du rhin 67402 Illkirch France See on map EU contribution € 0,00 Participants (3) Sort alphabetically Sort by EU Contribution Expand all Collapse all CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France EU contribution € 0,00 Address Rue charles sadron 45071 Orleans See on map NATIONAL UNIVERSITY OF IRELAND, GALWAY Ireland EU contribution € 0,00 Address University road 90 Galway See on map Links Website Opens in new window SARTORIUS AG Germany EU contribution € 0,00 Address Weender landstrasse 94-108 37075 Goettingen See on map
