Objective The project proposes a strategy aimed at increasing by an important factor the output of electrophoretic sequencing, mapping and DNA-probe genetic diagnosis, by consistently integrating innovations in different fields in which the proposers have specific competences. The most important of those innovations, each of which represents a valuable intermediate target, are: -New improved permanent and non-permanent sieving matrices for sequencing and duplex DNA separation -Design of an automated capillary array electrophoresis apparatus able to load from Microtiter plates, and to perform constant-field and pulsed-fields electrophoresis. -Alternative strategies using neutral molecules or particles bound to DNA to increase band separation. -Improved injection protoeols for capillary electrophoresis to avoid prepurifieation steps and increase automation -Comprehensive predictive models to accelerate the optimization of sequencing and DNA assay using pulsed-fields and/or polymer solutions. The project corresponds to area III.5 of the call for proposals, and more partieularly to the tasks: "development of new or improved sequencing approaches..." and "application of new methods of DNA probe diagnosis and their automation..." The whole projeet will be driven by fundamental studies including: -Theoretical modelling and computer simulations -Extensive studies of molecular migration mechanisms and of collective behavior by fluorescence microscopy. This approach, which requires multiple acute competences but no dramatic and hypothetical technlogieal breakthrough, would provide a rather secure and short route to accelerated sequencing and mapping, and automatised diagnosis. The chances of success of the project are maximised by exploring several parallel solutions for the most critical aspects (e.g. sieving matrices, increase of resolution). Fields of science natural sciencesbiological sciencesgeneticsDNAsocial sciencessociologyindustrial relationsautomationnatural sciencesphysical sciencesopticsmicroscopynatural scienceschemical scienceselectrochemistryelectrophoresisnatural sciencesmathematicsapplied mathematicsmathematical model Programme(s) FP3-BIOMED 1 - Specific research and technological development programme (EEC) in the field of biomedicine and health, 1990-1994 Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator Centre National de la Recherche Scientifique (CNRS) Address 10 rue vauqelin 75231 Paris France See on map EU contribution € 0,00 Participants (3) Sort alphabetically Sort by EU Contribution Expand all Collapse all CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France EU contribution € 0,00 Address Avenue albert schweitzer - chateau brivazac 33600 Pessac See on map Istituto Nazionale per la Fisica della Materia Italy EU contribution € 0,00 Address 93,via fratelli cervi 20090 Milano - segrate See on map ROWETT RESEARCH INSTITUTE United Kingdom EU contribution € 0,00 Address Greenburn road AB21 9SB Aberdeen See on map Links Website Opens in new window
