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Content archived on 2024-05-15

Structure and function in macromolecules

Objective

The aim of this study is the construction of a model of the immunocomplex between the MIR decapeptide and anti-AChR autoantibodies So that these data may be used for the design of high affinity therapeutic antibody fragments. The known NMR structure of the MIR decapeptide, when bound to the antibody molecule, will be used for docking experiments to the known crystal structures of Fab198 and Fab192. The crystal structures of Fab192 and Fab198 subsequently wiII be used in conjunction with the molecular modeling structures of two other anti-AChR monoclonal antibodies (Fab35 and Fab195), in order to combine elements of their structure with known characteristics of their function, The above experiments will show the nature of the interaction between the monoclonal antibodies and the antigen (AChR) and important residues for this reaction wiII be revealed. 3. OBJECTIVES AND EXPECTED ACHIEVEMENTS a. Models of Fab 35 and Fab195 based on homology modeling experiments. b. Docking of the Fab molecules to the known NMR structure of the MIR decapeptide c. Determination of antibody residues with important structural and functional importance d, Design of mutant molecules with possibly higher affinity for human AChR

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ACM - Preparatory, accompanying and support measures

Coordinator

POULAS KONSTANTINOS
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Total cost

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