Objective
EC cells are pluripotent cells that are widely used to study the regulation of gene expression during early developmental stages. EC cells can be induced to differentiate into a variety of cell types by retinoic acid (RA), by aggregate formation and by expression of the adenoviral 12S E1A oncoprotein. The coactivator p300 has been implicated in RA- and E1A-induced differentiation but the factors or activities that are downstream of p300 remained unclear. The Stunnenberg's lab have recently identified a novel basal factor, TAC, and provided firm evidence that TAC is a downstream target and effector of p300 activity. TAC is a stable transcriptional complex between TBP and TFIIA, it lacks classical TAFs, and is present and active in EC but not in differentiated cells. The acetyltransferase activity of p300 is crucial for the formation of TAC. Importantly, expression of p300 in differentiated cells instigated the formation of TAC from endogenous proteins. In this project, I will set out to unravel two aspects of the p300-TAC connection, using a combination of biochemical, cell system and in vivo approaches:
(1) The role of p300 in TAC formation.
(2) The role of p300 in TAC-mediated transcription. Collectively, these experiments are likely to shed light on the putative role of p300 (CBP) and its downstream effector TAC in differentiation of embryonal cells and to provide clues a to the target genes of TAC as well as to the general mechanism of gene trancription.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
6500 HB NIJMEGEN
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.