Objective
Identifying target genes of signaling pathways that operate during embryogenesis is an important step toward understanding how signals govern morphogenesis. I propose to search for targets of Hedgehog signaling in the hope of understanding how Hedgehog controls the formation of segment boundaries in Drosophila embryos. I will also pay attention to Hedgehog targets that might be involved in Wingless degradation, another interest of my host lab. I will apply the degron technology to construct a temperature sensitive allele of cubitus interuptus an essential effector of Hh signaling. RNA extraction after temperature shifts and hybridization onto micro arrays will allow me to uncover manges in transcriptional profile caused by loss of cubitus interuptus function and hence identify physiological target genes that operate at different stages. If the degron method cannot be applied to Drosophila, I will use the inducible expression of two forms of cubitus interuptus (a constitutive repressor and a constitutive activator) to identify targets. In site hybridization will be used to confirm candidate targets and subsequent functional analysis will assess the cell biological function of confirmed targets.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences developmental biology
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences genetics RNA
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NW7 1AA London
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.