Objective
oskar ( osk) mRNA encodes the Drosophila posterior determinant and its correct localization in the oocyte is essential for embryonic development. oskar mRNA is synthesized in the nurse cells during early oogenesis and transported and anchored to the posterior pole of the oocyte by a mechanism that is still unclear. Understanding the mechanism of osk mRNA movement during oogenesis requires the identification of the key determinants of its transport at the molecular level. I propose to develop an assay based on the analysis of the movement of osk RNA particles at high resolution by time-lapse confocal microscopy. osk mRNA transport will be visualized in living egg chambers after injection of fluorescent labeled RNA, as well as in vitro, in a reconstituted system. These assays, combined with biochemistry and genetics will be used to identify: i) nuclear components responsible of osk mRNA exit from the nurse cell nuclei; ii) osk RNA interacting proteins which could define the localization of osk in the oocyte cytoplasm during the different steps of Drosophhila oogenesis; iii) cellular proteins which could link osk mRNA to the cytoskeletal network: iv) cytoskeletal motors required for ask active transport through the egg chamber and v) the cis-acting signals present in osk mRNA involved in its transport.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences developmental biology
- natural sciences physical sciences optics microscopy confocal microscopy
- natural sciences biological sciences genetics RNA
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
HEIDELBERG
Germany
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