Objective
We aim to investigate iron homeostasis in the model organism C. reinhardtii. Ferritin, a 24-multimeric complex involved in iron storage and located in the chloroplast, is a key component to regulate iron intracellular concentration. We first will construct strains that over or under express ferritin. For this we will take advantage of the existence of a ferritin cDNA, that will be inserted in both orientations under the control of an HSP70 promoter. Nuclear transformation will allow the insertion of the constructs into the genome of a recipient strain. We will compare the phenotype of the resulting strains with wild-type strains grown under different iron concentrations I mainly at two levels. The expression of certain genes that are known to respond to iron concentration will be investigated by Northern Blot. The chloroplast protein content will be monitored by two-dimensional gel electrophoresis, and proteins that display a differential expression will be analyzed by mass spectrometry. Protein network involved in chloroplast iron homeostasis or ferritin regulation will then be identified. Our goal is to dissect ferritin function in chloroplast relevant and in general aspects of iron homeostasis. The proposed research project will allow me to develop new competencies on the Biochemistry of the model organism C. reinhardtii (chloroplast proteome analysis by 2D electrophoresis and mass spectrometry, protein complex purification using FPLC). I will also learn nuclear transformations which complete my experience in chloroplast transformation. I think that having a broad and integrated knowledge on many aspects of C. reinhardtii Biology will be a great advantage in my carrier. The host group will take benefit of my strong background in Molecular Biology and my knowledge in Genetics.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences chemical sciences electrochemistry electrophoresis
- natural sciences chemical sciences analytical chemistry mass spectrometry
- natural sciences biological sciences genetics genomes
- natural sciences biological sciences molecular biology
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
PF 07740 JENA
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.