The proposal is to develop a novel chemical system that allows the chemistry on the inside of a vesicle membrane to be coupled to a selective molecular recognition event on the outside of the membrane, but without direct physical transport of any chemical entities across the membrane. This will allow the development of a module system where the recognition units on the outside of the membrane can be varied at will and the nature of the chemical signal generated on the inside of the membrane can be amplified and tailored for a variety of different applications. A simple prototype system has been developed in the Hunter lab, and the key objectives for this proposal are
1. to develop an enzymatic system that will transduce and amplify the chemical signal generated on the inside of the vesicles
2. to explore the scope of possible external recognition head groups that will interface effectively with the transduction system. The project will provide excellent training for the applicant, who will learn and improve a wide range of skills including organic synthesis, bio-organic synthesis, membrane chemistry, spectroscopy, analysis and molecular modeling. In addition, the applicant will have the opportunity to work in a multidisciplinary group that is a leader in supramolecular chemistry and molecular recognition in the UK, and this will be particularly important for the applicant's future career. Significant breakthroughs in new areas of science are increasingly coming from a multi-disciplinary approach to research, and well-trained scientists with a background in inter-disciplinary research will be at the forefront of these developments in the future.