Skip to main content

Training network on functional genomics and proteomics of arabidopsis ubiquitin ligases and their targets in signalling

Objective

After completion of yeast, Arabidopsis, Caenorhabditis, Drosophila and human genome sequences, the next challenging task of biology is to identify the functions of eukaryotic genes and their protein products, which is a prerequisite for future biotechnological applications. Building upon its traditional agricultural links, plant research can now deploy new research tools to functionally characterise the Arabidopsis and rice genomes using proteomics and functional genomics. As the function of genes is ultimately controlled by the stability of their protein products, a central target is to understand how the ubiquitination-dependent proteolysis pathways regulate the stability of proteins. In these pathways, E3 ubiquitin ligases SCF (Skp1-cullin-F-box protein) and APC/C (anaphase promoting complex) are essential to control the stability of regulatory factors involved in signal transduction, transcription and cell division. Due to applications in cancer biology, the study SCF and APC/C is a focus of intense current research. In Arabidopsis, about 2.5% of all proteins represent SCF and APC/C components suggesting that SCF and APC/C mediated proteolysis represents also a central regulatory pathway in plants. The goal of this proposal is to gain a generally applicable information about the function and regulation of conserved components of plant SCF and APC/C complexes. To reach this goal, a network will unite interdisciplinary expertise to study the composition and substrates of SCF and APC/C complexes using protein interaction, biochemical, mass spectrometry, physical and immunological techniques, reverse genetics, transcript profiling and high-through-put genetic engineering. The project aims to reach a break-through in our understanding of the role played by plant SCF and APC/C complexes in the regulation of cell division and signalling.

Funding Scheme

NET - Research network contracts
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Address
Rue De Général Zimmer 12
67084 Strasbourg
France

Participants (5)

ALBERT-LUDWIGS-UNIVERSITAET FREIBURG
Germany
Address
1,Schaenzlestrasse 1
79104 Freiburg (In Breisgau)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
Avenue De La Terrasse 1Bètiment 23
91190 Gif Sur Yvette
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
Germany
Address
Carl-von-linné-weg 10
50829 Koeln
ROYAL HOLLOWAY AND BEDFORD NEW COLLEGE
United Kingdom
Address
Egham Hill
TW20 0EX Egham,surrey
UTRECHT UNIVERSITY
Netherlands
Address
Padualaan 8
3584 CH Utrecht