Objective
Foreseen Results
As soon as candidate growth and development-related genes will have been identified, sense and antisense constructs will be introduced into Malus, Prunus and Vitis plants, using transformation procedures already in use in participating laboratories. After initial characterization of transgene copy number and expression features, transgenic woody plants showing increased or decreased expression of the genes of interest will be selected and micropropagated. Using the same tissue culture conditions that will have been used to select development-related genes, these transgenic plants will be scored for morphological modifications. After transfer to greenhouse conditions, these plants will also be screened for growth and development alterations in vivo.
Many of the regulatory circuits underlying plant growth and development processes still remain to be discovered and understood. However, it has become increasingly clear that basic signal perception and transduction mechanisms (involving cytoplasmic calcium ions) and their associated biochemical cascades play a key role in these phenomena, like in many others. From this point of view, the regulation of tree growth and shape constitutes one of the most challenging issue, not only because of its apparent complexity, but also because of its major impact on many agricultural or forestry problems.
The development of plant molecular biology techniques has allowed to isolate tools that can now be used to understand, at the molecular level, some of these processes. Apple tree DNA probes, corresponding to genes whose products are likely to be involved in plant growth and development regulation, will be used to dissect molecular events related to the perception and transduction of developmental or morphogenetic signals. This will be achieved by taking advantage of in vitro tissue culture models that are available for Malus and Prunus species. At the same time, RNA fingerprinting methods will be used to extend the list of available probes. The transgenic aequorin system will also be used, in order to collect data concerning cytoplasmic Ca2+ level variations in response to specific signals in these woody plants. This will help to question the relationship between observed variations in specific gene expression levels and signalling pathways.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- agricultural sciences agriculture, forestry, and fisheries agriculture horticulture arboriculture
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences molecular biology
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
5030 Gembloux
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.