Objective
The main objectives are as follows:
* To map quantitative trait loci which are segregating in pedigrees which have been specially constructed by crossing susceptible zebu with trypanotolerant N'Dama.
* Typing of associated markers in populations which consist of purebred African trypanotolerant cattle
* Typing of associated markers in populations which are crossbred in origin but which have been under strong trypanosomiasis challenge.
* A search for DNA sequence polymorphisms in markers and functional genes in candidate regions will be conducted and these will be similarly typed and analysed in hybrid populations
* Verified markers will be typed in a series of Togolese cattle populations which have been characterised for a series of trypanotolerance-defining parameters. The power of trypanotolerant markers in predictive epidemiology will be assessed.
Expected Outcome
Trypanosomiasis is a major constraint to livestock production in Africa. However, some of the indigenous Bos taurus populations of cattle are markedly resistant to the effects of the disease. The trait responsible, termed trypanotolerance, is heritable and quantitative in nature but its genetic basis is unknown. The identification of chromosomal regions which contain major genes determining trypanotolerance would be a valuable contribution to African agriculture: - aiding selection programmes, conservation measures and adding to the basic scientific understanding of disease resistance, possibly leading to the development of entirely novel disease control approaches. This project will identify such regions, verify them using independent analyses, and refine the map position of the trait loci. An assessment of their importance in the field will also be conducted. Close collaboration between DCs and European institutes, together with personnel exchanges will lead to training and technology transfer, and a strengthening of the scientific base in the DCs.
The key activities envisaged are:
* Sampling and DNA extraction of animals from a range of population in each of Senegal and Togo, choosing locations of high trypanosomiasis challenge.
* Preliminary population typing of these samples with diagnostic microsatellites and selection of four hybrid and two pure taurine populations
* Further sampling of dam/offspring pairs from these. Establishing of DNA sample banks
* Typing of pedigree DNA samples with a genome-wide panel of markers.
* Identification of chromosome regions containing trypanotolerance loci.
* Identification of further markers and/or sequence polymorphisms which reside in these regions, typing of these in both hybrid and pure taurine, trypanotolerant populations. Also typing of the same markers in reference populations for comparison. Deduction of composite haplotype frequencies and levels of admixture at candidate regions in the test populations.
* Analysis of evidence of reduced introgression of zebu introgression at candidate region loci in hybrid populations. Analysis of variance of microsatellite length in pure taurine populations.
* Epidemiological analyses
* Personnel exchange between project partners. Publication of data and results in both traditional and electronic format.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences health sciences public health epidemiology
- agricultural sciences agriculture, forestry, and fisheries agriculture
- agricultural sciences animal and dairy science domestic animals animal husbandry
- natural sciences biological sciences genetics chromosomes
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
2 DUBLIN
Ireland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.