Objective
The main objectives are as follows:
* To enlarge the knowledge of reproductive cells isolated from rice, with particular emphasis on their cellular and molecular aspects.
* To develop methodologies for introducing foreign DNA in sperm cells isolated from rice.
* To develop and standardize procedures for "in vitro" fertilization using isolated gametes.
* Construction of "gamete banks" for different varieties of rice plants.
* Regeneration of rice plants by "in vitro" fertilization using isolated gametes.
* Generation of transformed rice plants, with improved characteristics of agronomical interest.
Expected Outcome
The work carried out in this project should provide new techniques for gamete isolation from different rice varieties. The creation of a cDNA library of rice varieties and the identification of specific gene products for the sperm or egg cells is also expected. As a final goal the programme should lead to the introduction of genes of agronomic interest into sperm cells in order to obtain improved rice plants.
The key activities envisaged are:
* Detailed cytological and ultrastructural investigation on anthers, pollen grains and pollen tubes, stigma, style and embryo sac either by Transmission Electron Microscope or Confocal Laser Scanning Microscope.
* Establishment of experimental conditions for the isolation and culture of gametes.
* Isolation of mRNA from sperm cells and construction of cDNA libraries.
* Investigation on isolated male and female gametes with particular emphasis on their cellular, biochemical and molecular aspects.
* Development of "in vitro" fertilization techniques using isolated gametes.
* Investigation on fusion products (zygotes), with reference to the cytoplasmic organization.
* Transformation of gametes by insertion of foreign DNA.
* "In vitro" fertilization using transformed gametes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences physical sciences optics microscopy
- medical and health sciences clinical medicine embryology
- natural sciences physical sciences optics laser physics
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
53100 Siena
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.