* To identify physiological traits that explain submergence tolerance and intolerance in rice.
* To identify genetic markers for tolerance to complete submergence in flood water in rice.
* To identify links between submergence tolerance and drought tolerance.
The research is expected to have two principal outcomes. Firstly, the basic understanding of the physiological basis of tolerance and intolerance to complete submergence in rice should be improved. This work should identify features that may help plant breeders select for submergence tolerance with greater ease and confidence. Secondly, the genetic map of markers for tolerance is expected to help construct a marker-based selection scheme for plant breeders to use in selecting tolerant lines in various national breeding programmes.
* To screen and rank the 118 double haploid lines for submergence tolerance under both outdoor and laboratory conditions where the environment is closely monitored. These lines are the outcome of a cross between a submergence tolerant parent (FR13A) and a susceptible parent (CT 6241-17-5-1).
* To screen and rank the double haploid lines for physiological attributes that may be related to the differences in submergence tolerance. These attributes include anoxia tolerance, carbohydrate levels, ethylene responsiveness, constitutive porosity, activity of fermentation and glycolytic enzymes.
* To gain a deeper understanding of the physiology of submergence tolerance through detailed studies of interconnectednesss of internal gas spaces, underwater photosynthesis, substrate re-allocation and distribution, effect of development on sensitivity to submergence, photosynthetic efficiency, volatile production during post-anoxic stress, tolerance to submergence by roots.
* To test the extent of negative or positive interactions between drought and submergence tolerance.
* To create a high density chromosome map that locates sections of one or more chromosomes that carry the gene or genes for submergence tolerance.
* To make preliminary tests of selected double haploid lines for agronomic and submergence tolerance in real farming situations in Asia.
Funding SchemeCSC - Cost-sharing contracts
73140 Nakhorn Pathom
25150 Bansang - Prachin Buri
6500 GL Nijmegen