Objective
The main objectives are as follows:
* The mapping of genes responsible for aluminium tolerance in maize by using genetic and molecular methods.
* The identification of genes associated with aluminium tolerance in maize and analysis of their expression.
* The identification of Arabidopsis ecotypes tolerant and sensitive to aluminium and analysis of the modification of the rhizosphere.
* Production of transgenic plants altered in the expression of genes involved in the biosynthesis of organic acids.
* Analysis of specific root epidermis promoters in transgenic tobacco and maize plants.
Expected Outcome
The project intends to provide tools for the analysis of aluminium tolerance in maize such as molecular markers and cDNA clones corresponding to genes induced or repressed in different aluminium conditions. A screening in a model plant, Arabidopsis thaliana, will be carried out in order to obtain tolerant and sensitive ecotypes. Transgenic tobacco and maize plants with altered organic acid biosynthesis will be obtained and they will be analyzed for aluminium tolerance. Transgenic plants will also be used to analyze promoters specific for root epidermis.
The key activities envisaged are:
* Mapping of maize genes involved in aluminium tolerance using available segregant populations and molecular marker methodology.
* Identification of genes involved in aluminium tolerance by differential display and differential screening between tolerant and sensitive maize lines in the presence or absence of aluminium. Analysis of the structure and expression of selected clones.
* Production of transgenic and tobacco plants overexpressing bacterial citrate synthase and overexpression of PEPC and MDH. Analysis of variations in organic acid production and secretion in root exudates. Study of the tolerance of the transgenic plants to aluminium.
* Screening of Arabidopsis thaliana ecotypes for aluminium sensitivity. Analysis of root exudates and initial genetic analysis.
* Analysis of promoters encoding root-specific malic enzyme in tobacco and maize and analysis of the specificity of expression in the epidermis.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences organic chemistry organic acids
- natural sciences chemical sciences inorganic chemistry post-transition metals
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
08034 Barcelona
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.