Objective
The main objectives are as follows:
* To assess the importance of catalase for the cellular defence against environmental stress using a catalase-deficient barley mutant.
* To analyse the sequence of molecular events that occur during abiotic stress in a catalase-deficient barley mutant.
* To characterise the antioxidant defence response in maize against drought stress and in rice against salt and pathogen stress.
* To establish genetic transformation methodology of barley.
* To produce transgenic maize, rice and barley with increased levels of catalase and to assess stress tolerance of transgenic versus control lines.
Expected Outcome
The work carried out in this project should identify the role of catalase in the defence against various stress factors and will address the feasibility of improving stress tolerance by increasing the levels of catalase in monocotyledonous plants. Comparison of oxidative damage and catalase overproduction during stress in C3 and C4 plants will contribute to elucidating the role of photorespiration in photooxidative processes evoked by environmental adversity. Establishment of a barley transformation and regeneration system will be highly valuable for technology-based improvement of this important crop.
The key activities envisaged are:
* Expression analysis of antioxidant defence proteins during environmental stress in maize, rice and barley will be assessed by measuring enzyme activities and mRNA levels. Partial cDNAs of the major antioxidant genes will be cloned by RT-PCR to be used as probes in the mRNA analysis. Antioxidant gene expression will also be followed in catalase-deficient barley in order to understand the molecular mechanisms that are activated specifically by H2O2 stress. Low molecular weight antioxidants such as ascorbate and glutathione will be measured by HPLC with simultaneous UV and electrochemical detection.
* Development and/or improvement of methodologies: barley transformation using Agrobacterium tumefaciens, barley regeneration from immature embryos, methods for monitoring H2O2 and oxidative damage (lipid peroxidation, protein carbonylation).
* Construction of expression cassettes for catalase overproduction in maize, rice and barley and transformation of these monocotyledonous species. Analysis of stress tolerance against drought, salt stress and pathogens. Salt stress will be imposed in hydroponic cultures, pathogens include viruses, bacteria and fungi.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences microbiology mycology
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Coordinator
9000 GENT
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.