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Application of biotechnology for genetic improvement of oilseed rape

Objective

The main objectives are as follows :
* To identify the loci controlling oil content in Chinese and European cultivars of oilseed rape.
* To develop molecular tags for a self-incompatibility system to provide breeders in China and Europe with a new production system and a broader genetic base for hybrid varieties.
* To identify sets of genes for resistance to all known strains of Turnip Mosaic virus (TuMV).
* To transfer the TuMV resistance genes from Brassica rapa (turnip rapeseed) into B. napus (oilseed rape) and B. oleracea (broccoli, cauliflowers and European cabbages).
Expected Outcome

The chief scientific innovations will be in the areas of quantitative genetics and interspecific gene transfer. The proposed research will be a pioneering investigation into the genetic control of seed oil accumulation in Brassica crops. The application of marker-assisted selection will help to maintain the efficiency of endogenous plant breeding programmes in China. This material will lead to the development of improved crop varieties through plant breeding and these improved varieties will be of benefit to Chinese and European agriculture. Varieties with increased oil content will help to satisfy domestic consumption and will produce a better return for the farmer. Varieties with natural resistance to TuMV will produce a more stable yield and reduce the requirement for chemical control methods. F1 hybrids based on the SI system will reduce the dependence on Polima CMS and increase the security of the Chinese oilseed rape crop.
The key activities envisaged are :
* Mapping of Quantitative Trait Loci (QTL) in populations of doubled haploid lines by use of RFLP markers. The influence of the contrasting environments of China and Europe on the effects of the oil content QTLs will be investigated.
* Molecular tags for S-alleles and other tags for genes that suppress self-incompatibility (SI) will be developed. Microsatellites will be used to identify combinations with large genetic distance.
* Identification of RAPD markers specifically associated with TuMV. Establishment of two segregating populations fully characterised for TuMV resistance. Production of amphidiploid B. rapa/B. oleracea interspecific hybrids to transfer resistance genes.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

GEORG-AUGUST-UNIVERSITY GOETTINGEN
Address
Von-siebold-strasse 8
37075 Goettingen
Germany

Participants (6)

CHINESE ACADEMY OF AGRICULTURAL SCIENCES
China
Address
30,Bai Shiqiao Road 30
100081 Beijing
DNA Landmarks Inc.
Canada
Address

J3B 6Z1 St-jean-sur-richelieu - Quebec
HORTICULTURE RESEARCH INTERNATIONAL
United Kingdom
Address
Wellesbourne
CV35 9EF Warwick
Huazhong Agricultural University
China
Address
Shizhishan, Wuchang
430070 Wuhan - Hubei
SVALOF WEIBULL AB
Sweden
Address

268 81 Svaloev
Zhejiang Academy of Agricultural Sciences
China
Address
198,Shiqiao Road
310021 Hangzhou