Objective
The main objectives of the project are as follows :
* Establishment of cell suspension cultures from the YELLOW SIGATOKA-resistant somaclonal Cavendish mutant CIEN BTA-03 and its susceptible mother plant BRASILERO.
* Development of an analytical HPLC method for Musa phytoalexins and a DNA marker
system to measure (semi)quantitatively the response of Musa cells after elicitation by crude elicitor preparations of both pathogenic Mycosphaerella species.
* Application of the newly developed marker system(s) to select the fungal crude preparation with the highest elicitation activity, and to characterize Mycosphaerella isolates from different geographical origins for their elicitation potential.
* Systematic use of M. fijiensis and M. musicola elicitors to discover differences of gene expression patterns between the resistant CIEN-BTA-03 mutant and the corresponding mother plant BRASILERO.
* Use of M. fijiensis and M. musicola elicitors to analyse differentially expressed cDNAs in the Musa acuminata ssp. burmanicoides (CALCUTTA IV), a wild BLACK SIGATOKA-hypersensitive banana, to isolate a fast fungus-inducible promotor.
* Design and assembling of Musa promotor/reporter gene constructs, stable transformation of susceptible banana cultivars, and in vivo testing of the reporter gene induction by local Mycosphaerella populations in greenhouses.
Expected Outcome
The main goal of this project is the isolation of several fungus-inducible Musa defense gene promotors for genetic engineering of BLACK/YELLOW SIGATOKA resistance in Musa cultivars. Novel data on the induction of different banana defense genes by a range of fungal elicitor preparations and the time course of their transcriptional activity will be gathered. A Musa BAC library will be available for future use (e.g. cloning of resistance genes). Finally, a transgenic Musa cultivar with improved resistance to M. fijiensis and M. musicola is expected.
The key activities envisaged are :
* Elicitation of Yellow - and Black Sigatoka-resistant and -susceptible plants, RNA-isolation and cDNA synthesis, performance of differential display RT-PCRs and subtractive hybridizations to isolate and characterize differentially expressed cDNAs.
* Establishment of a BAC library from a resistant banana and use of differentially expressed cDNAs to isolate corresponding Musa defense genes, and to characterize their promotor sequences.
* Assembling of promotor/reporter gene constructs, stable transformation of susceptible banana cultivars, sampling and single-sporing of M. fijiensis and M. musicola from Latin America and infection of transgenic banana plants using conidiospores.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences medical biotechnology genetic engineering
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
60439 Frankfurt-am-Main
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.