* To carry out an international multicentric population-based study of occupational factors of laryngeal cancer.
* To organize an international conference on occupational and environmental cancer in developing countries, with emphasis on Latin America.
The study will assess the contribution of occupational exposure to an important neoplasm in the study areas. It will contribute original information on the interaction between genetic factors, known risk factors, such as tobacco and alcohol, and occupational exposures in laryngeal cancer formation.
The proposed approach is a multicentric case-control study of exposure to occupational and other environmental risk factors, taking into account also HPV infection, genetic susceptibility and mutations in critical genes. The study will be conducted in Rio de Janeiro, Sao Paulo, Pelotas and Porto Alegre (Brazil), Montevideo (Uruguay) and Buenos Aires (Argentina). It will include approximately 1200 cases of laryngeal cancer and a similar number of controls. A detailed occupational questionnaire will be used to interview cases and controls, and will then be interpreted on an individual basis by a team of local experts, which will assess exposure to a list of known or suspected occupational laryngeal carcinogens in terms of probability, frequency and level. The preliminary list of exposures to be assessed includes asbestos, man-made mineral fibres, wood dust, strong inorganic acid mists, diesel engine exhaust, environmental tobacco smoke, other sources of PAHs, chromium, nickel, arsenic, formaldehyde, infection with HPV, infection with animal viruses. A blood sample will be collected, whenever feasible, from cases and controls. Laryngeal tumour samples will be collected, whenever feasible, from cases. The analysis of genetic polymorphism to GST M and NAT2 enzymes will be performed on DNA extracted from lymphocytes. Tumour samples of a subgroup of cases selected according to relevant exposures (e.g. exposed and unexposed to occupational carcinogens) will be analysed for mutations in the p53 and ras genes using denaturing gradient gel electrophoresis.
The target audience of the Conference are researchers in the field of occupation and environmental health and professionals involved in occupational health. Among the topics to be covered are: state of the art in occupational and environmental cancer research; industrialization and health; burden of occupational and environmental cancer in developing countries; transfer of hazardous technology and patterns of exposure to occupational and environmental carcinogens in developing countries. The Conference will be held in Rio de Janeiro on 30 July - 1 August 1998.
Funding SchemeCSC - Cost-sharing contracts
22241-210 Rio De Janeiro
1417 Buenos Aires
01246 São Paulo