The applicants have recently developed an innovative method to improve Rhizobium-Legume biological nitrogen fixation, plant biomass and seed production, by introducing into Rhizobium two bacterial genes coding for a second pathway for the synthesis of insole-3-acetic-acid (IAA) . In greenhouse trials temperate and tropical legumes increase stem biomass by 50-75% and seed production by 50-100% when modified and unmodified strains are compared. The objectives of the present application are :
1) to evaluate the yield increase in field trials for Soybean, Peanut, Vilna, Vetch and Pea;
2) to monitor the ecological impacts of releasing genetically engineered Rhizobia;
3 ) to obtain the same yield increase by using two wild type strains (one nitrogen fixing, one releasing IAA);
4) to reduce the use of major environmental pollutant as chemically synthesised nitrogen fertilisers by promoting legumes as intercrop.
Funding SchemeCSC - Cost-sharing contracts
50200 Sansai,chiang Mai