Objective
The initial stage of organic toxicants degradation in plants is their oxidative transformation. As it is well known, this is rate limiting in multi-step complicated process of detoxification. According to different considerations xenobiotic oxidation in plants is carried out via monooxygenase, or via peroxidase, or via phenoloxidase. On the basis of our investigations, we can suppose that none of the stated mechanism is not universal way for xenobiotic detoxification. Consequently, the hypothesis is drawn out, according to which, the interchange of oxidative systems in xenobiotic detoxification takes place, which should be determined by many internal and external factors.
The importance of this research is to determine exactly what enzymatic systems participate in the first step of xenobiotics oxidation - hydroxylation and to establish the mechanism of their coordination.
The goal of the research is the establishment of possible pathways of nitrobenzene oxidation in soybean and maize plants; the reveal of the fact of different oxidative enzyme systems interchange in xenobiotic hydroxylation.
To achieve the Goal following tasks will be addressed:
Effect of xenobiotic on cell ultrastructure.
Xenobiotic oxidation by monooxygenase, peroxidase and phenoloxidase enzymatic systems in subcellular fractions.
Quantitative input of each enzymatic mechanisms in xenobiotic oxidation.
The intensity of oxidation processes in relation with plant cultivation regime and age. Variants: etiolated plant seedlings; seedlings grown in light; seedlings infected with pathogenic fungus; seedlings grown on xenobiotic containing solutions; different age seedlings.
The expected results will contribute for more clear understanding of the mechanism of interchange, whether it is realized: by different oxidation enzymes interchange each other; by the modification of cytrochrome P450 activity; or by the combination of the two mechanisms.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
31931 Toulouse Cedex 9
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.