Livrables
Results :
The problem of environmental pollution is a growing one in Russia due to problems inherited from the former Soviet Union. Of particular concern is the vast range of petrochemical pollutants that contain the polycyclic aromatic hydrocarbons (PAHs). These pose a major ecological and human health hazard and excavation and off-site treatment of such wastes is difficult and undesirable. Thus in situ methods of dealing with such wastes has become a favored alternative. This relies upon optimisation of the microbialdegradation processes in soils. The objective of the project was to investigate the efficiency of polycyclic aromatic hydrocarbon (PAH) degradation by soilmicroorganisms and their genetically modified derivates in polluted soils by a study of survival, stability, competitiveness and biodegradative activity. The progress of practical work associated with the project was largely the responsibility of researchers in the laboratory at Pushchino with some assistance from the other participants. A number of model systems andanalytical methods for the study of PAH biodegradation by microorganisms insitu in soils. New PAH-degrading strains were obtained. The stability, competitiveness and survival of a number of key microorganisms was determined; particularly comparing indigenous and introduced microflora in model soil systems. In addition, the genetic control of PAH biodegradation in tthe most active strains was determined. Overall the work has added a realistic assessment of the potential of a range of microorganisms to mediate PAH biodegradation under the conditions likely to prevail in nature. For example changes in moisture content of soils has a profound effect on the effectiveness of naphthalene degrading bacteria.
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