Objective
Interaction between ion transporting membrane proteins and lipids of the membrane-forming bilayer is assumed to be a general mechanism of protein regulation. To understand the involved mechanisms the project proposes to study the effects of two principal characteristics of the lipid bilayer, the distribution of the electric field in the membrane dielectric and the membrane elasticity, on the function of mechano-sensitive channels and the Na,K-ATPase.
The alteration of the membrane parameters will be caused by adsorption of lipophilic, membrane-active substances (styryl dyes as modificators of the dipole potential, amphiphilic ions, phospholipase), inorganic ions of high affinity to phospholipids (lanthanides) and ions with different chaotropic effects on the membrane surface.
It is planed to measure and analyse quantitatively characteristic properties of membranes of different lipid composition with different techniques on planar lipid bilayer (measurements of boundary potentials, charge relaxation and conductivity in the presence of hydrophobic ions, transversal membrane compressibility), on lipid monolayers (Volta potentials and pressure-area diagrams) and on liposome suspensions (electrokinetic studies, calorimetry, fluorescence). These approaches will be used to obtain information on the structure of ion-conducting pathway of Na,K-ATPase. The effects of local electric fields on the kinetics of charge transfer will be studied by electric measurements on lipid bilayer modified by fragments of cell membrane using the methods developed by authors of the project and by spectral measurements on free fragments with fluorescent dyes.
The results of the studies will be applied to develop physical models of ion transport by Na,K-ATPase and of blocking effects of lanthanides on the mechanosensitive channels.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Data not available
Coordinator
78434 Konstanz
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.