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Content archived on 2022-12-23

RNA-cleaving strong binding antisense oligonucletide conjugates

Objective



The collaborating laboratories will develop antisense oligonucleotide constructs of a new generation, which will form very tight complexes with their target RNAs and will be capable of catalytically cleaving the target RNAs. Synthetic organic compounds mimicking catalytic centers of nucleases will be designed.
These will be the compounds bearing imidazole residues, carboxylic, guanidino- and amino groups. The RNA cleaving molles will be conjugated to oligonucleotide analogs capable of tight binding to specific target sequences: strong binding oligonucleotide analogs with modified bases and DNA aptamers tageted to specific elements of RNA structure.
Approaches will be developed to design antisense oligonucleotides for difficult RNA targets, which will be oligonucleotides capable of invading into the RNA structure. The developed oligonucleotide conjugates and methods will be applied to design of specific and efficient antisense oligonucleotide conjugates targeted to RNAs of important pathogens: viroids, ribosomal RNA of bacteria and mycoplasma, genomic and messenger RNA of influenza virus.

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Programme(s)

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Call for proposal

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Funding Scheme

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Coordinator

Institut fuer Biochemie
EU contribution
No data
Address

97074 Wuerzburg
Germany

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Total cost

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Participants (3)

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