Objective
The present project is aimed at design of efficient approaches to targeting different DNA and RNA hairpins with oligonucleotide conjugates and with new rationally designed organic molecules capable of interacting with elements of hairpin structures.
Loose hairpin structures in natural viral RNAs and messenger RNAs will be identified using combinatorial approaches based on oligonucleotide libraries and PCR techniques. Structure and stability of these hairpins and different known hairpins of biological significance present in viral RNAs and eucaryotic mRNAs will be investigated by chemical and enzymatic probing and by physico-chemical methods. Novel organic ligands interacting with elements of nucleic acid hairpins will be designed:
compounds of cyclo-bis-intercaland type which are expected to recognize heterocyclic bases in loops,
"molecular wedges", planar molecules capable of forming multiple hydrogen bonds with pairs of heterocyclic bases located at a distance allowing the "molecular wedges" to insert themselves between the bases.
Approaches for targeting specific hairpin structures with these compounds alone and in combination with oligonucleotides, reactive derivatives of oligonucleotides and multicomponent oligonucleotide compositions will be developed.
These studies are expected to result in design of new specific ligands capable of unfolding and chemically modifying certain hairpins and ligands capable of stabilizing certain hairpins and modulating functions of specific nucleic acids. These ligands will find applications in researches aimed at investigation of structure and functions of nucleic acids and in development of nucleic acid related analytical methods and nucleic acid targeted therapeutics.
Results of the studies will be presented as research papers.
Fields of science (EuroSciVoc)
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
67000 Strasbourg
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.