Objective
The overall objective of the proposal is to understand the biochemistry and chemistry of methane oxidation as catalysed by pMMO. It also has significant value as a carbon and energy source for the production of bacterial biomass as a source of animal feed. Methane-oxidizing bacteria can also be used for the production of proteins, nucleic acids and chemical components from them (amino acids, nucleotides, nucleosides etc.), enzymes, lipids, as a raw materials for production of pharmaceutical preparations. Living biomass may be used for decreasing of methane in mines. It is MMO, that is the most efficient catalyst for the direct production of methanol from methane at ambient temperature and pressure. pMMO has evolved over billions of years to harness methane to methanol and can tell us precisely how Nature has solved the problem. This information can also be used for rational design of small inorganic catalysts. We wish to:
(1) Investigate continuous cultivation of the methanotrophs Methylococcus capsulatus (strain M), Methylococcus capsulatus (Bath) and Methylosinus trichosporium OB3B for controlling synthesis of highly active pMMO;
(2) Investigate paths of electron transfer from different reducing agents to O2-binding site of pMMOH and possible links to ATP synthesis and the electron transport chain;
(3) To investigate the dependence of methane monooxygenation in methanotrophs on ion gradients across the membrane and components of H+
(4) Determine the general properties of active pure pMMOH, including the 3D structure and the structure and properties of the active sites by different methods ;
(5) Obtain structural information on the nature of the metal-ion and its ligands in the active site of highly active pMMOH and
(6) By genetic engineering produce new commercial stable edible high yielding strains and stabilized the enzyme for detailed structural mechanistic studies. This information can further be used for production and design of small inorganic or biological catalysts.
The project bring together the leading European scientists in the methanotrophic field in a real integrated complimentary project designed to train young scientist and produce safe, cheap and clean feed protein as well as novel efficient catalyst.
This is economic important field of research as seen by the fusion in 2003 of Norferm A/S (Norway and Denmark) with the Dupoint (large parts in USA), they will produce "Bioprotein" from mainly these freeze-dried bacteria and the dry North Sea gas. Similar plans for restarting the earlier large production of bacterial biomass from natural gas as protein source is in progress in NIS.
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Programme(s)
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Coordinator
0316 Oslo
Norway
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