Objective
The overall objective of the research project is the development and application of a new molecular strategy to turn the hidden biodiversity of marine bacteria into novel biotechnological products. This strategy is based on an integrated molecular approach using bacterial DNA and RNA obtained directly from the marine environment to circumvent the need for cultivation since only a very small fraction, usually less than 1%, of the marine bacteria can be cultured. The molecular approaches used are gene cloning and expression of marine bacterial genes, estimation of the structure of bacterial communities that carry the genes in situ, and molecular characterization of bacterial isolates. This integrated approach will be applied to a set of diverse marine habitats to obtain information about i) the diversity of microbial genes in marine habitats and their carrying bacteria, and ii) the expression systems necessary to make the genetic information available for biotechnological products such as enzymes and antibiotics. The diversity of the microbial community will be determined with rapid molecular profiling techniques (5S rRNA analysis and denaturing gradient gel electrophoresis of 16S rDNA) that are able to give an quantitative overview about the abundant species in the community. A set of different cloning vector will be used to generate polygenomic clone libraries directly from the marine habitat.
Expression of these genes will be attempted by different expression vectors followed by automated activity measurements in an industrial high-through-put unit. These activity determinations and molecular analysis in combination with microbiological and biotechnological studies will result in: i) commercial products, i. e. a variety of novel psychrophilic and mesophilic enzymes, and ii) development of an intelligent strategy for the assessment of microbial diversity in any natural environment and its biotechnological potential, i. e. how and where to look with what molecular approaches for which products.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology microbiomes
- natural sciences biological sciences genetics RNA
- natural sciences chemical sciences electrochemistry electrophoresis
- natural sciences biological sciences microbiology bacteriology bacteriomes
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
38124 Braunschweig
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.