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Content archived on 2024-04-16

COORDINATION OF RESEARCH ON VARIABILITY OF HUMAN IMMUNO- DEFICIENCY VIRUS (HIV)

Objective


Human immunodeficiency viruses (HIV) exhibit a high degree of genetic polymorphism which results in widespread antigenic variability and in different patterns of virus infection and cytopathogenicity in culture and possibly in vivo. Whether and how these features influence the course of acquired immune deficiency syndrome (AIDS) is basically unknown. It is thus mandatory to elucidate the role played by the viralheterogeneity in determining the course of the disease and its variable pattern in different geographical areas of the world. It may also be important to know whether AIDS patients exhibit a double or possible multiple infection by several HIVs and how this modifies the features of the syndrome.

Variability is being studied at genomic, antigenic and biological levels. In each of these three aspects coordination of all European efforts and possibly collaboration of the participating Institutions is essential and profitable. In order to study the molecular determinants at the basis of the biologic characteristics of different isolates phenotypically distinct, but genetically highly homologous, molecular clones are generated and characterized. Genomic changes are also investigated by selected analysis of HIV proviruses. The study of HIV defective variants, either present in natural isolates or induced isolates will allow possible correlation of genomic and phenotypic viral features with a modified course of HIV infection in vitro. The study of antigenic levels will include mapping of immunodominant epitopes on structural and regulatory viral proteins in different isolates. The localization of epitopes on the envelope glycoproteins that elicit neutralizing antibodies in humans is a prerequisite for the development of immunogens capable of inducing protective immunity in man. Relevance of possible results will obviously reside in setting the stage for a realistic rationale approach the vaccine problem, in expanding the potentialities of diagnostic tests, and th e knowledge of HIV biology and pathogenesis.

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Programme(s)

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Funding Scheme

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CON - Coordination of research actions

Coordinator

Head of the Unit of Foodborne Zoonoses and Veterinary Epidemiology
EU contribution
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Total cost

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