Objective High resolution analysis of gene expression can be performed by RNA in situ hybridization, which allows definition of gene expression with great accuracy, this revealing temporal gene activity in individual cells or groups of cells. To apply the power of this high-resolution procedure to the vast resource of cDNAs contained in the evergrowing public sequence databases, seven European groups decided to undertake a concert effort in: a) developing a plan for international coordination on gene expression studies; and b) carrying out a high-throughput expression study of a large number of genes of high biological interest in developing mouse and human embryos as well as in adult mouse brain.The main objectives of this proposal are:1) Select and retrieve a collection of genes of biological value based on extensive bioinformatic analysis of public sequence database. This selection will be based on an extensive bioinformatic analysis of public sequence databases, such as dbEST and UniGene.2) Perform a high-throughput expression study of the genes identified in objective 1, in developing mouse and human embryos and in adult mouse brain. The high-throughput in situ hybridization studies will be conducted by using an automated in situ hybridization (ISH) procedure, under development by the applicants. Using these procedures, 2,000-6,000 murine and 200-400 human cDNAs will be analyzed in the course of this project.3) Construct two project-specific database (Mapped Image Database and Archive Image Database) for the submission, storage, management and release to the scientific community of the expression data generated in this project.The high-throughput RNA in situ hybridization analysis described in this proposal will:- be of crucial importance to better understand the physiological role of a given gene/protein in a living organism;- provide new insights into mammalian gene functions and investigate their possible role in inherited diseases;- be instrumental for predicting the phenotypic consequences of gene disruption in mouse mutants;- provide insight into gene product interactions or indicate hierarchies of gene expression networks. Fields of science natural sciencescomputer and information sciencesdatabasesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsRNAmedical and health sciencesclinical medicineembryology Programme(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Topic(s) 1.1.1.-8. - Research into genomes and diseases of genetic origin Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator FONDAZIONE TELETHON Address Via pietro castellino 111 80131 Napoli Italy See on map EU contribution € 0,00 Participants (5) Sort alphabetically Sort by EU Contribution Expand all Collapse all MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Germany EU contribution € 0,00 Address Hofgartenstrasse 8 Muenchen See on map Links Website Opens in new window MEDICAL RESEARCH COUNCIL United Kingdom EU contribution € 0,00 Address Crewe road western general hospital EH4 2XU Edinburgh See on map UNIVERSIDAD MIGUEL HERNANDEZ DE ELCHE Spain EU contribution € 0,00 Address Avenida del ferrocarril s/n 03202 Elche See on map UNIVERSITE DE PARIS V 'RENÉ DESCARTES' France EU contribution € 0,00 Address Rue de l'ecole de medecine 12 75270 Paris See on map UNIVERSITY OF NEWCASTLE UPON TYNE United Kingdom EU contribution € 0,00 Address NE1 7RU Newcastle upon tyne See on map
