Objective
Transcription factors (TF) are recognised as having a great biotechnological potential and also to have played a pivotal role in the evolution of plants.
The aim of this project is to establish the function of plant TFs, and it will involve:
(i)the definition of TF gene expression patterns in Arabidopsis;
(ii) the identification of insertion mutations at TF loci;
(iii) the ectopic expression of TFs (or derivatives) in Arabidopsis and in crop plants;
(iv) their phenotypic analysis;
(v) the analysis of interactions between TFs, and
(vi) the generation of a bio-informatics infrastructure.
This programme will help to reveal the full biotechnological potential of plant TFs and provide insights into hierarchies, redundancies, interdependencies and into their evolution. The study will involve the preparation of a TF gene array and of a normalised TF ORF library in a 2-Hybrid vector, two tasks justifying the European dimension of the project.
Second edition of the TF gene array, containing 1194 probes and 26 controls..81 Arabidopsis lines containing constructs over-expressing TFs and 28 RNA interference/antisense RNA constructs.·7 tomato lines transformed with TF over-expressors, RNAi or antisense constructs. ·4 rapeseed lines transformed with TF over-expressors, RNAi or antisense constructs. ·Improved methods for high-throughput metabolic and AFLP-based RNA profiling. ·Library of 125 insertion mutants at TF loci. ·Web accessible database bringing together information on TFs, their gene sequences, and interactions, with custom-built navigation and interrogation tools.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences databases
- natural sciences biological sciences genetics mutation
- agricultural sciences agriculture, forestry, and fisheries agriculture
- natural sciences biological sciences genetics RNA
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NR4 7UH NORWICH
United Kingdom
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Participants (22)
79104 Freiburg
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67056 LUDWIGSHAFEN
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91190 GIF SUR YVETTE
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28049 MADRID
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9000 GENT
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37073 GOETTINGEN
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06466 GATERSLEBEN
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75341 Paris
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NORWICH
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50829 KOELN
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10589 BERLIN
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00185 ROMA
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WAGENINGEN
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RE42 6ET BRACKNELL
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2 DUBLIN
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20122 MILANO
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28040 MADRID
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BS41 9AF BRISTOL
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LS2 9JT Leeds
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00185 ROMA
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75105 UPPSALA
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3584 CH UTRECHT
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