As unicellular organisms, bacteria must match their metabolic activity to the requirements of the environment. This is achieved by regulatory networks that respond to intra-and extra-cellular signals by modulating gene expression. This proposal aims to generate new data on the regulation of gene expression in bacillus subtilis using state-of-the-art technologies for analysing the proteome and transcriptome. The information will be constructed, in silico, into a model of the global regulatory networks that result from interactions between individual regulatory circuits. It will make full use of bioinformatical and physical resources generated by the european bacillus community during previous sequencing and functional analysis projects. The data will provide new strategies for the identification of gene function and will provide clues to general stress mechanisms induced in pathogenic bacteria in response to infection. A major outcome will be an innovative transcriptional database, subscript, that is inter-linked to existing genomic, proteomic and phenotypic databases, to provide one of the most complete descriptions of living organism.
Funding SchemeCSC - Cost-sharing contracts
9750 AA Haren Gn